Characterisation of Plasmodium vivax lactate dehydrogenase dynamics in P. vivax infections

P Cao; S Kho; MJ Grigg; BE Barber; KA Piera; T William; JR Poespoprodjo; IK Jang; JA Simpson; JM McCaw; NM Anstey; JS McCarthy; S Britton

Journal article

Characterisation of Plasmodium vivax lactate dehydrogenase dynamics in P. vivax infections

P Cao, S Kho, MJ Grigg, BE Barber, KA Piera, T William, JR Poespoprodjo, IK Jang, JA Simpson, JM McCaw, NM Anstey, JS McCarthy, S Britton

Communications Biology | NATURE PORTFOLIO | Published : 2024

DOI: 10.1038/s42003-024-05956-6

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Abstract

Plasmodium vivax lactate dehydrogenase (PvLDH) is an essential enzyme in the glycolytic pathway of P. vivax. It is widely used as a diagnostic biomarker and a measure of total-body parasite biomass in vivax malaria. However, the dynamics of PvLDH remains poorly understood. Here, we developed mathematical models that capture parasite and matrix PvLDH dynamics in ex vivo culture and the human host. We estimated key biological parameters characterising in vivo PvLDH dynamics based on longitudinal data of parasitemia and PvLDH concentration collected from P. vivax-infected humans, with the estimates informed by the ex vivo data as prior knowledge in a Bayesian hierarchical framework. We found th..

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University of Melbourne Researchers

Pengxing Cao Author

Julie Simpson Author

James McCaw Author

James McCarthy Author

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Funding Acknowledgements

We thank Dr Sophie Zaloumis (University of Melbourne) for helpful conversations about the method for model fitting and Dr Louise Marquart (University of Queensland) for her preliminary analysis of the VIS data. We also thank Dr Gonzalo Domingo (PATH) for his contribution to data collection and analysis. The work was supported by the National Health and Medical Research Centre (NHMRC) of Australia: Project Grant (1025319), a Senior Principal Research Fellowship to Nicholas M. Anstey (1135820), Investigator Grants Leadership Level to James McCarthy (GNT2016396) and Julie A Simpson (1196068) and supported in part by the Australian Centre for Research Excellence on Malaria Elimination (1134989). Steven Kho is supported by a Menzies Future Leaders Fellowship.